Category Archives: Science

Isolation and multiplex analysis of six polymorphic microsatellites in the Antarctic notothenioid fish, Trematomus newnesi

J. K. J. VAN HOUDT, B. HELLEMANS, A. VAN DE PUTTE, P. KOUBBI, F. A. M. VOLCKAERT
Molecular Ecology Notes Volume 6, Issue 1, pages 157–159, March 2006
molecular-ecology-notes

Six polymorphic microsatellites were isolated from the dusky notothen, Trematomus newnesi (Boulenger 1902), using a microsatellite enrichment protocol and selective hybridization with di- tetra- and penta-repeat probes. The loci were screened in 48 individuals captured in the Southern Ocean (coastal zone of Terre-Adélie), revealing eight to 22 alleles per locus and an observed heterozygosity ranging from 0.70 to 0.92. These microsatellite markers provide a tool to study the relationship between the various morphs observed in this species and can be used for population genetic analysis and biodiversity studies.